Molecular Stress Physiology

The department focuses on the analysis of the physiological and metabolic responses of crops to abiotic stress and how the knowledge gained from these studies can be employed to enhance the tolerance of such crops to stressful conditions. Translational approaches (from model species to crops, from crop to crop) will form a constitutional basis of the research conducted by the group. One of the research lines followed by the Department is identification and characterization of genes involved in modulating oxidative stress responses and reactive oxygen species (ROS)-induced programmed cell death. Recently, a team lead by Assoc. Prof. Dr. T. Gechev identified a novel plant-specific gene that regulates the tolerance to oxidative and abiotic stresses (Sujeeth et al. 2019). As this gene has homologs in major crops, this finding may have an impact on the management of stress tolerance in crop species and increasing their productivity under unfavorable environmental conditions.

Fig. 1. Principle of plant defense activation by priming. Treatment with priming agents (left) induces endogenous plant defense mechanisms (middle), leading to subsequent stress tolerance in the primed plants. Agrochemicals are predominantly applied as a uniform spray solution (broadcast application) that can easily result in chemical waste, excessive carryovers, and damage to the vegetation. In contrast, directed application targets individual plants. Technological advances, such as computer vision and machine learning, make directed application more efficient than ever. Specific spraying of individual plants allows reduction of the dosage that can eliminate up to 90% of the used chemical and mitigate its environmental impact. Another exciting technology that will soon be available to the farmers combines agrochemicals with nanobodies as formulation agents that specifically target leaves or pests, improving retention limiting the input of chemicals for the environment.

A third research focus of Dr. Gechev’s team is the resurrection species Haberlea rhodopensis. This plant is tolerant not only to desiccation but also to other environmental stresses such as long-term darkness and low temperatures. The team studies molecular mechanisms of tolerance to these abiotic stresses, using “-omics” technologyes such as transcriptomics, metabolomics, and lipidome analysis (Durgud et al. 2018).

Head of Department:
Assoc. Prof. Dr. Tsanko Gechev	gechev@cpsbb.eu
Members:
Dr. Aakansha Kanojia	kanojia@cpsbb.eu
Dr. Nikola Staykov	staykov@cpsbb.eu
Dr. Ramadoss Dhanushkodi	dhanushkodi@cpsbb.eu
Ludmil Tonchev	ltonchev@cpsbb.eu
Mihail Angelov	angelov@cpsbb.eu

Publications:

Kerchev P., van der Meer T., Sujeeth S., Verlee A., Stevens C.V., Van Breusegem F., Gechev T. (2020) Molecular priming as an approach to induce tolerance against abiotic and oxidative stresses in crop plants. Biotechnology Advances. https://doi.org/10.1016/j.biotechadv.2019.107503.

Lyall R., Gechev T. (2020) Multi-omics insights into the evolution of angiosperm resurrection plants. Annual Plant Reviews Online. 3: 1–34.

Omidbakhshfard M.A., Neerakkal S., Gupta S., Omranian N., Guinan K.J., Brotman Y., Nikoloski Z., Fernie A., Mueller-Roeber B., Gechev T. (2020) A biostimulant obtained from the seaweed Ascophyllum nodosum protects Arabidopsis thaliana from severe oxidative stress. International Journal of Molecular Sciences. 21(2): 474. https://www.mdpi.com/1422-0067/21/2/474/pdf.

Dong Y., Gupta S., Sievers R., Wargent J., Wheeler D., Putterill J., Macknight R., Gechev T., Mueller-Roeber B., Dijkwel P. (2019) Genome draft of the Arabidopsis relative Pachycladon cheesemanii reveals novel strategies to tolerate New Zealand's high Ultraviolet B radiation environment. BMC Genomics. 20(1): 838

Gupta S., Dong Y., Dijkwel P., Mueller-Roeber B., Gechev T. (2019) Genome-wide analysis of ROS antioxidant genes in resurrection species suggest an involvement of distinct ROS detoxification systems during desiccation. International Journal of Molecular Sciences. 20(12): 3101. https://doi.org/10.3390/ijms20123101.

Sujeeth N., Mehterov N., Gupta S., Qureshi M.K., Fischer A., Proost S., Omidbakhshfard M.A., Obata T., Benina M., Staykov N., Balazadeh S., Walther D., Fernie A.R., Mueller-Roeber B., Hille J., Gechev T.S. (2019) A novel seed plants gene regulates oxidative stress tolerance in Arabidopsis thaliana. Cellular and Molecular Life Sciences. pp 1–14. http://doi.org/10.1007/s00018-019-03202-5.

Durgud M., Gupta S., Ivanov I., Omidbakhshfard A., Benina M., Alseekh A., Staykov N., Hauen-stein M., Dijkwel P.P., Hortensteiner S., Toneva V., Brotman Y., Fernie A.R., Mueller-Roeber B., Gechev T. (2018) Molecular mechanisms preventing senescence in response to prolonged darkness in a desiccation–tolerant plant. Plant Physiology. 177(3):1319–1338. https://doi.org/10.1104/pp.18.00055.

Petrov, V., Qureshi, M. K., Hille, J., Gechev, T. (2018) Occurrence, biochemistry and biological effects of host-selective plant mycotoxins. Food and Chemical Toxicology. 112:251–264. http://doi.org/10.1016/j.fct.2017.12.047.